Attention is given to the design of the experiment, which aids in showing clear evidence of biochemical features that may otherwise escape notice
Bridges theory of ligand binding and allostery with experiments Applies historical and physical insight to provide a clear understanding of ligand binding Written by a renowned author with long-standing research and teaching expertise in the area of ligand binding and allostery Based on FEBS Advanced Course lectures on the topic Reversible Ligand Binding Theory and Experiment is an ideal text reference for students and scientists involved in biophysical chemistry, physical biochemistry, biophysics, molecular biology, protein engineering, drug design, pharmacology, physiology, biotechnology, and bioengineering.
Classical experiments are reviewed in order to further highlight the importance of the design of the experiment
Overall, the book supplies students with the understanding that is necessary for interpreting ligand binding experiments, formulating plausible reaction schemes, and analyzing the data according to the chosen model s
Special consideration is devoted to enzymology, a field usually treated separately from ligand binding, but actually governed by identical thermodynamic relationships
Topics covered include theory of ligand binding to monomeric proteins practical considerations and commonly encountered problems oligomeric proteins with multiple binding sites ligand binding kinetics hemoglobin and its ligands single-substrate enzymes and their inhibitors two-substrate enzymes and their inhibitors and rapid kinetic methods for studying enzyme reactions